International Journal of Multidisciplinary Research and Development

International Journal of Multidisciplinary Research and Development


International Journal of Multidisciplinary Research and Development
International Journal of Multidisciplinary Research and Development
Vol. 7, Issue 1 (2020)

Analytical method development and validation of diclofenac sodium by UV-visible spectroscopy using AUC method


N Karnakar, H Ramana, P Amani, D Sri Tharun, M Nagaraju, Saurabh Bodhgaya Sharma

A simple, precise, accurate and economical UV visible spectrophotometric method has been developed for estimation of Diclofenac sodium drug by AUC method. The standard and sample solutions were prepared by using methanol as a solvent. Quantitative determination of the drug was performed at wavelength range 250-350 nm. The linearity was established over the concentration range of 10, 20, 30, 40&50 µg/ml for Diclofenac sodium with correlation coefficient value of 0.997. Precision studies showed that % relative standard deviation was within range of acceptable limits. The mean percentage recovery was found to be 99.38%. The proposed method has been validated as per ICH guidelines.
Pages : 20-24