Identification of intra- and inter-specific variations in Passiformes of Kabul province using COX1 mitochondrial gene
Mohammad Sharif Arianmal, Hedayatullah Salari, Mansour Aliabadian
The objective of this study was to assess interspecific and intraspecific variation of Afghan passiformes birds by extracting DNA from the COX1 mitochondrial gene. In the case of this family of birds, it has been thought that is a single group of mono-descendent origin. However, new studies have shown that many passiformes families are not common in traditional classifications, and a more complete understanding of the phylogeny of passiformes can only be achieved by extensive molecular studies. Sampling were done from ten (stations during three periods in 2015 and 2016 in Kabul province included Deh sabz, Bagrami, Sarobi, khak-i-Jabbar, Shakar-daraeh, Gol Dareh, Paghman, Char- asiabe, Road Andarabi and Kafroshi). 190 samples were collected using Miss net and 148 samples were transferred to the laboratory. Of these, 110 specimens of passiformes and 38 non- passiformes samples are in the sample. The hunted birds were related to 13 families, 23 genera and 35 species. The family of Emberizdae with 7 species the most diverse and the family of Passeridae with the highest number of 45 individduals. After morphometry, the specimens were transferred to the laboratory for molecular studies, and DNA extraction and sequencing of the COX1 gene were performed to identify the species and to study the molecular distances. From 7704 pairs of intra-species genetic variations were, variations ranged from zero to six, while from 814700 pairs of inter-species genetic variations, the variation was greater than zero to 25.