The effect of the maternal induced diabetes on the postnatal development of the dorsomedial nucleus in hypothalamus of the albino rat.
Mohammed NM Saleh, Sayed AS Hassan, Faten Y Mahmoud, Merry B
Maternal diabetes constitutes an unfavorable environment for embryonic and postnatal development. The aim of the present work was to detect the effect of maternal induced diabetes on the structural organization of the dorsomedial nucleus during postnatal development. Hypothalamic specimens of the offspring of diabetic mothers were studied at ages ofnewborn, 21 days and two months. Several techniques were used in this work including, gallocyanine-chrom-alum staining technique, Golgi –Cox method and immunohistochemistry. In addition, morphometric study was done to estimate the number of neurons in the dorsomedial nucleus. In the newborn, some neurons in the dorsomedial nucleus showed the presence of darkly stained nuclei. With the progress of age these degenerative changes became more prominent. Marked reduction in the extension and branching of dendrites were observed. In addition, an apparent increase in the number of astrocytes was found. Measuring the number of cells per area demonstrated the presence of a highly significant decrease in number at the ages of 21 days and two months in the offspring of diabetic mothers in comparison with the control group. In conclusion, this study indicated that maternal diabetes affect the development and structure of the dorsomedial nucleus. These changes suggest that there should be strict control of diabetes before and during pregnancy.