Vol. 4, Issue 1 (2017)
Studies on endophytes and antibacterial activity of Plumbago zeylanica L
Author(s): Anand Sagar, Madhu Bala, Ved Prakash
Abstract: Plumbago zeylanica L. is a medicinal plant commonly known as chitrak, belonging to family Plumbaginaceae. In the present investigation, plant parts of Plumbago zeylanica were collected to isolate the endophytes and to analyse the antibacterial activity. Thirteen species of endophytic fungi belonging to eight genera (Aspergillus, Curvularia, Fusarium, Penicillium, Pythium, Rhizopus, Sporotrichum and Trichoderma) were isolated from leaves, stem and roots of P. zeylanica during summer, rainy and winter seasons. The antibacterial activity of methanol, acetone and ethanol stem and root extracts of P. zeylanica was determined in vitro against medically important pathogens (i.e. Escherichia coli, Staphylococcus aureus and Yersinia pestis) following Agar-well diffusion method using different concentrations (25%, 50%, 75% and 100%). Results showed low to significant antibacterial activity against the mentioned bacterial strains. Methanol leaf extract was found to be more effective against selected pathogenic bacteria as compared to acetone and aqueous leaf extracts.