International Journal of Multidisciplinary Research and Development

ISSN Online: 2349-4182
ISSN Print: 2349-5979

Vol. 3, Issue 6 (2016)

Plant regeneration of an anti-diabetic plant: Stevia rebaudiana L. Bertoni and evaluation of its antimicrobial activity using in vivo leaf extracts

Author(s): Sunita Murmu, Anjana Bhagat, Souvagyalaxmi Sahoo
Abstract: A convenient and faster protocol established for clonal propagation of Stevia rebaudiana Bertoni through callus and shoot culture, with its antimicrobial screening. MS medium with (0.5-2.0mg/L) BAP was found to be optimal for shoot initiation. A high-frequency multiplication rate was obtained from nodal segments in MS medium supplemented with 0.5 mg/l BAP+2.0 mg/l Kn. Root induction occurred with IBA 1.0 mg/l then transferred to poly tray containing sand, vermiculite and soil (1:1:2) and acclimatized for a period of 3 weeks. In vivo grown leaf extracts in different solvent system exhibited a concentration dependent antibacterial and antifungal inhibition against standard by disc diffusion method. The methanolic extracts of Stevia leaf showed best antibacterial and antifungal activity against a number of microorganisms. This proved Stevia’s potential as antimicrobial agent from non antibiotics sources. Therefore, commercial manufacture of active constituents from these improved elite lines would be useful and profitable.
Pages: 144-148  |  1021 Views  338 Downloads
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