Abstract: Jatropha curcas L., belonging to Euphorbiaceae family, has attained global attention for its innumerable commercial utilities and as a biofuel crop. The plant can be grown with minimal inputs in arid and semi-arid environments for the production of biodiesel from its seeds. Non-availability of quality planting material calls for an urgent requirement of identification and production high yielding selections or hybrids. Further, there is also a requirement of rapid multiplication of identified superior selections or hybrids. In the present study, micropropagation of clonal/F1 hybrid seeds through embryo culture is carried out to standardize an in vitro protocol for rapid multiplication. Embryos from clonal/F1 hybrid seeds were germinated in MS basal medium with 0.1 mg/L BAP and were multiplied up to three cycles in medium containing 1 mg/L BAP. The plants obtained were rooted in medium containing 0.5 mg/L IBA and successfully acclimatized. The efficacy of embryo culture of J. curcas for mass multiplication is discussed.