International Journal of Multidisciplinary Research and Development

International Journal of Multidisciplinary Research and Development

Online ISSN: 2349-4182
Print ISSN: 2349-5979

Vol. 2, Issue 5 (2015)

Influence of culture conditions on cellulase production by sclerotium rolfsii sacc

Author(s): Amit Kumar Chaurasia, Shridha Chaurasia, Subha Chaurasia, Sushmita Chaurasia
Abstract: The purpose of this study was to determine the influence of culture media, incubation period, temperature and pH on the cellulase (cx) enzyme production by Sclerotium rolfsii. Activity of cellulase enzyme was assayed by viscometric method. In order to select the best suitable medium, the production of cellulase enzyme was estimated by growing the Sclerotium rolfsii in ten different culture media for 9 days at 30º C temperature. Among ten culture media, Asthana and Hawker’s, Czapek’s, Glucose-dox and Glucose-nitrate broth media have not favoured the production of cellulase enzyme as in the culture filtrate of these four media, no trace of cellulase enzyme have been detected. While in other remaining six media (i.e. Basal mucor, Brown’s, Dextrose- asparagine phosphate, Elliot’s, Fernando’s and potato dextrose) Sclerotium rolfsii was able to produce cellulase enzyme. Amongst these six media, potato dextrose broth medium was found to be the best for the production of cellulase enzyme. For further study, potato dextrose broth medium was selected for cellulase enzyme production. The production of cellulase enzyme has been studied at different incubation period. The Sclerotium rolfsii was cultured at 30ºC on potato-dextrose broth medium for 3, 6, 9, 12, 15 and 18 days. It was found that Sclerotium rolfsii has a capability of producing cellulase enzyme within short incubation period i.e. 3 days incubation. Production of cellulase enzyme, gradually increased with increasing the length of incubation period up to 9 days and further increase in length of incubation up to 18 days, did not show any effect on the production of cellulase. The nine days incubation has found to be the best incubation period for the maximum production of cellulase enzyme. The influence of different temperatures on the production of cellulase enzyme was also investigated by growing the Sclerotium rolfsii at various temperatures viz., 15º, 20º, 25º, 30º, 35º, 40º and 45ºC. The Sclerotium rolfsii was able to produce the cellulase enzyme at wide range of temperature, ranging from 15º to 35ºC. The 30ºC temperature was found to be optimum for the maximum production of cellulase enzyme. The effect of different hydrogen ion concentrations (pH), viz., pH 3.0, 4.0, 5.0, 6.0, 7.0, 8.0 and 9.0 were also tried on the production of cellulase enzyme. The isolated pathogen Sclerotium rolfsii able to synthesized cellulase enzyme on a wide range of pH, i.e., from pH 3.0 to 9.0. Extremely acedic pH (i.e., at pH 3.0), the activity was found to be less which increased gradually with increase in pH up to pH 5.0. Further increasing the pH, has no effect on the production of cellulase enzyme. The pH 5.0 was found to be optimum for the maximum
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