The worldwide prevalence of diabetes mellitus has risen dramatically in the developing countries over the past two decades. Most of the worldwide rise is thought to be type 2 diabetes linked to the â€œmetabolic syndromeâ€ â€“ the cluster of metabolic perturbations that includes dyslipidemia, hypertension, and insulin resistance. Regular screening of adults is essential for early detection and care. India is the shelter of number of people with diabetes mellitus. Allahabad district, (Uttar Pradesh) has very limited studies on Diabetes mellitus awareness and prevalence in this district. A cross-sectional study on Prevalence and assessment of knowledge was done. This study on adults and elderly age in the different area of Allahabad was taken with structured questionnaire used to assess the knowledge of diabetes and Random blood sugar test done to detect diabetes. The prevalence of Diabetes mellitus was highest in George Town area i.e. 14.3% due to sedentary life style and lowest in Muthiganj area i.e. 07.00% due to moderate life style. But relevant knowledge about Diabetes is very in both areas. There is very little data on the level of awareness and prevalence about diabetes in developing countries like India. Such data is very important to plan the public health programs and Health camps. This study was taken up to identify, investigate and evaluate knowledge and practice through exploratory and evaluatory research.