International Journal of Multidisciplinary Research and Development

International Journal of Multidisciplinary Research and Development

Online ISSN: 2349-4182
Print ISSN: 2349-5979

Vol. 2, Issue 11 (2015)

In Vitro Seed Germination and Protocorm Development of Geodorum densiflorum (Lam.) Schltr. An Endangered Terrestrial Orchid

Author(s): Theng PA, Korpenwar AN
Abstract: The mature capsule of Geodorum densiflorum was used as explant for present study. Seeds obtained from mature capsule were successfully germinated on two basal media MS and Kn C by using different combination of growth hormones. The various concentration of BAP and NAA were supplemented to media for better germination of seed and development of protocorm. 0.1% activated charcoal also added to media with plant growth regulators. The seed germination was observed on MS media and Kn C media. The seed germination on MS and Kn C media was 95.60 % and 92.74 % respectively after 120 days of inoculation. rnThe protocorm formation was assessed at up to the highest seed germination and protocorm formation seen in MS medium. The spherule formation was observed in 9- 12 weeks on both media after germination of seed. The white colored spherule was turned into sturdy green color after 12-15 weeks. Whitish, light yellow, brown and green colored protocorms were observed in developmental stages of protocorms.rn
Pages: 347-350  |  861 Views  345 Downloads
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