Vol. 2, Issue 10 (2015)
Production and Partial Purification of Xylanase from Bacillus pumilus
Author(s): Harsh Chaudhary, Veenu Chaudhary, Harit Kasana
Abstract: Microbial enzymes produced from bacteria and fungi are widely used in the industries because they are cheaper to produce and their enzyme contents are more predictable and reliable as compared to those from plants and animal sources. Hemicellulose is the second most abundant plant fraction available in nature, formed by pentose sugars. Hemicelluloses are made of xylan (xylose, arabinose and glucuronic acid) backbone. Xylan is the major constituent of hemicelluloses Present study was planned with the objective to optimize the production parameters of bacterial xylanases and partial purification of Xylanase enzyme. Bacillus pumilus showed maximum xylanolytic activity at 50 °C, time 48 hour and pH 7.0 ± 0.2. The peak enzyme activity was observed while supplementing xylose, as carbon source and ammonium nitrate (inorganic) and tryptone (organic) as a nitrogen source. Enzyme was extracted by centrifugation at 10,000 rpm for 20 minutes. Crude enzyme assay was done by DNS method. After partial purification total enzyme activity was found 35.78 Uml-1 and specific activity was 12.33 Umg-1. The pH and thermal stability of cellulose free xylanases are important issues for their potential industrial applications.