Vol. 2, Issue 10 (2015)
Pharmacognostic and Hptlc Studies on Oldenlandia Umbellata
Author(s): V. S. Saravanan, M. Mohamed Ismail, S. Manokaran
Abstract: Oldenlandia umbellata belongs to family Rubiaceae has been traditionally used as remedy against asthma and bronchitis. It is a small annual plant native to India and grows in tropical climate. Pharmacognostic studies and HPTLC profiles were carried out in the present study to ensure the authenticity and quality of oldenlandia umbellate. Microscopic observation of leaf of Oldenlandia umbellate revealed that the leaf is provided with the paracytic type of stomata. There are more number of stomata are present (46.3 ± 2.5) in the lower surface of the leaf whereas, no stomata were present on the upper surface. Transverse section of stem of Oldenlandia umbellata consist of outer most layers called Epidermis. Epidermis bears unicellular epidermal hairs. The transverse section of root consists of outer most layer of cork cells (Bark) followed by cork cambium. The HPTLC separation was achieved using N Hexane: Ethyl Acetate: Acetic Acid: formic acid (60:40:2.5:2.5) as the mobile phase. The ethanolic extract of oldenlandia umbellate showed sixteen different Rf values, of which the compound having Rf value 0.43 constitute more than 16% of total extract.