Vol. 2, Issue 10 (2015)
Cancer biomarkers detection using tracer nanoparticles and advanced nanoscopic scanning
Author(s): Arash Tahmasebifar, Kimia Tahani, Reza Ghanbari, Leila Haghshenas, Majid Shahbazi
Abstract: Background: New advances in the field of molecular medicine and the discovery of novel diagnostic and therapeutic approaches to cancer diseases will depend on identifying biomolecular structures and behaviors. Using advanced nano-detection devices, nanomedicine can provide a new insight into obtaining single-molecule analysis of functionalized biomolecules or biomarkers in human malignant disorders. Because of the critical biological functions of small non-coding RNA oligonucleotides, accurate nanoscopic identification of miRNA-21 molecule was supposed to be performed.rnMethods: To this end, gold nanorods (GNRs) as tracer particles with cationic surfaces were employed to label anionic miRNA-21 after normalization. Their successful interaction was approved by an average hydrodynamic diameter and surface zeta-potential test using dynamic light scattering (DLS). Then, the structural changes of the samples were investigated through scanning tunneling microscopy (STM) with a single atomic resolution. rnResults: The DLS results to investigate whether GNRs could efficiently label miRNA indicated affinity of CTAB-coated GNRs to miRNA-phosphate groups in the complexes. The high qualities of STM 2-D and 3-D nanographs of GNRs and GNRs-miRNA complexes demonstrated significant differences in morphologies, placing arrangements, surfaces, sizes, and aspect ratios. rnConclusion: Following the observed direct effects of the targeted biomarkers on the tracer gold nanoparticles, we introduced a new insight into biomedical agent nanocharacterization of molecular confrontation using a newer nanoscopic application. These findings can develop nanodiagnostic and prognostic techniques such as miRNAs detection in cancer diseases via advanced scanning nanotechnology and nanocolorimetric assay.