Vol. 2, Issue 10 (2015)
Standardization of proper dose of synthetic hormone for induced breeding of three loaches of genus Botia
Author(s): Arpita Dey, Sudip Barat
Abstract: This study describes the dosage of WOVA-FH for induced breeding of Botia sp. (Indian loach). Synthetic hormone, WOVA-FH was used to induce the species at different doses (0.5 ml/kg as 1st dose, 0.25 ml/kg as 2nd dosage, 0.025ml/fish as 3rd dose and 0.0125ml/fish as 4th dose) in each breeding trial. The higher fertilization, hatching and survival rates were found with fish injected with 0.025 ml/fish. High dose (0.25ml/kg) of hormone affected the fishes and finally perished within 4 days. Fishes which were injected with given 0.5 ml/kg succumbed within 2 days. Fishes that were given 0.0125 ml/fish dose of WOVA-FH injection moved and fed but did not spawn. Loaches required very little amount of synthetic hormone for breeding in captivity. The present study showed that 0.025 ml/fish of WOVW-FH was optimum sufficient for induced breeding of the three loaches Botia dario, Botia lohachata and Botia almorhae.