Vol. 2, Issue 10 (2015)
In-vitro Antimicrobial Activity and Cytotoxicity of Ethanolic leaves Extract of Acacia nilotica (L)
Author(s): Ahmed S. Kabbashi, Mohammed I Garbi, Elbadri A. Osman, Mahmoud M. Dahab, Waleed S. Koko, Nadir Abuzeid, Hamza A. A Salam, Ibrahim F. Ahmed
Abstract: Acacia nilotica (L) related to family Fabaceae-Mimosoideae. The division of Acacia nilotica is Magnolophyta and class is Magnolipsida. The genus is Acacia and species is nilotica. The ailments treated by this plant include colds, congestion, fever, gallbladder, hemorrhage, hemorrhoids, leucorrhoea, ophthalmic, sclerosis and small pox. Acacia bark is drunk for intestinal pains and used for treating acute diarrhea. Other preparations are used for gargle, toothache, ophthalmic and syphilitic ulcers. The present study was conducted to investigate antimicrobial (bacteria and fungi) and cytotoxicity (MTT assay) of ethanol extract of A. nilotica (leaves). The ethanol extracts of A. nilotica (leaves) was screened of antimicrobial activity against four standard bacteria, two Gram-positive bacterial strains (Bacillus subtilis and Staphylococcus aureus), two Gram-negative bacterial strains (Escherichia coli and Pseudomonas aeruginosa) and two fungal strains (Apergillus niger and Candida albicans) using the cup plate agar diffusion method. And screened for the cytotoxicity using 3- (4, 5-dimethyl thiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) by using Vero cell line. The ethanol extract of A. nilotica (leaves). exhibited inhibitory effects against most of the tested organisms with zone of inhibition ranging from (21-28 mm). And MTT assay verified the safety of the examined extract. In conclusion: These studies conducted for both A. nilotica in the treatment of several bacterial and fungal.